Part:BBa_J72038:Design

{Bmll7} in BBb

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

N/A

Source

PCR

Construction of MED21 Basic Part Bmll7

PCR MLL79/MLL80 on pBca9523-Bmll6 (405bp, EcoRI/BamHI)

Sub into pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+910, L)

Product is pBca9523-Bmll7

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MLL79 Forward Biobricking of Bmll7 CCATAGAATTCATGAGATCTatgACCGACCGTCTGACCC

MLL80 Reverse Biobricking of Bmll7 CGTTAGGATCCttaACCGTCAACGAAGTCTTCGATCAG